MA1-41201

antibody from Invitrogen Antibodies

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-41201

Provider

Invitrogen Antibodies

Product name

AKT3 Monoclonal Antibody (66C1247.1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

The sequence is identical in human, mouse, rat, cow, dog, and chicken. Suggested positive control: human kidney protein, antigen standard for AKT3 (transient overexpression lysate).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

66C1247.1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis for AKT3 using a AKT3 monoclonal antibody (Product # MA1-41201) at 2 µg/mL dilution against 30 µg/lane of human kidney lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AKT3 in 30 µg/lane human kidney lysate. Samples were incubated in AKT3 monoclonal antibody (Product # MA1-41201) using a dilution of 2 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of AKT3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Akt3 (66C1247.1) Mouse Monoclonal Antibody (Product # MA1-41201) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.