PA1-9004

antibody from Invitrogen Antibodies

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-9004

Provider

Invitrogen Antibodies

Product name

AKT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is predicted to react with bovine and canine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AKT3 in paraffin embedded Human Heart. Samples were incubated with AKT3 polyclonal antibody (Product # PA1-9004) using a dilution of 5 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AKT3 in human prostate. Samples were incubated with AKT3 polyclonal antibody (Product # PA1-9004) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AKT3 in paraffin embedded Human Prostate. Samples were incubated with AKT3 polyclonal antibody (Product # PA1-9004) using a dilution of 5 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AKT3 in human heart. Samples were incubated with AKT3 polyclonal antibody (Product # PA1-9004) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Samples were incubated with AKT3 polyclonal antibody (Product # PA1-9004) using a dilution of 10 µg/mL for 1hr followed by Alexa Fluor 488 secondary antibody at a dilution of 1 µg/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.