Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKT Pan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HA-human Akt -transfected 293T cells.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 whole cell lysate/extract of HA-human Akt-transfected 293T cells.7.5 % SDS-PAGE. Akt1/2/3 antibody AKT Pan Polyclonal Antibody (Product # PA5-31915) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1/2/3 antibody (Product # PA5-31915) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Human esophageal cancer tissue sections using AKT Pan Polyclonal Antibody (Product # PA5-31915).