PA5-31915

antibody from Invitrogen Antibodies

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31915

Provider

Invitrogen Antibodies

Product name

AKT Pan Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HA-human Akt -transfected 293T cells. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (99%), Cat (98%), Pig (99%), Chicken (100%), Sheep (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Akt1/2/3 antibody detects Akt1/2/3 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 whole cell lysate/extract of HA-human Akt-transfected 293T cells.7.5 % SDS-PAGE. Akt1/2/3 antibody AKT Pan Polyclonal Antibody (Product # PA5-31915) dilution: 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1/2/3 antibody (Product # PA5-31915) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining of Human esophageal cancer tissue sections using AKT Pan Polyclonal Antibody (Product # PA5-31915).