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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKT Pan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HA-human Akt -transfected 293T cells. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (99%), Cat (98%), Pig (99%), Chicken (100%), Sheep (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1/2/3 antibody (Product # PA5-31915) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1/2/3 antibody (Product # PA5-31915) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Human esophageal cancer tissue sections using AKT Pan Polyclonal Antibody (Product # PA5-31915).
