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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-64232 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ferroportin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKAGLKEEET ELKQLNLHKD TEPKPLEGTH LMGVKDSNIH ELEHEQEPTC ASQMAEPFRT FRDGWVSYYN
- Concentration
- 0.41 mg/mL
Submitted references Interleukin-6 triggers toxic neuronal iron sequestration in response to pathological α-synuclein.
The Ferroxidase Hephaestin in Lung Cancer: Pathological Significance and Prognostic Value.
Sterling JK, Kam TI, Guttha S, Park H, Baumann B, Mehrabani-Tabari AA, Schultz H, Anderson B, Alnemri A, Chou SC, Troncoso JC, Dawson VL, Dawson TM, Dunaief JL
Cell reports 2022 Feb 15;38(7):110358
Cell reports 2022 Feb 15;38(7):110358
The Ferroxidase Hephaestin in Lung Cancer: Pathological Significance and Prognostic Value.
Zacchi P, Belmonte B, Mangogna A, Morello G, Scola L, Martorana A, Borelli V
Frontiers in oncology 2021;11:638856
Frontiers in oncology 2021;11:638856
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Ferroportin Polyclonal Antibody (Product # PA5-64232) and a 63kDa band corresponding to Ferroportin was observed in all the tested tissue models. Whole cell lysate (30ug lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SLC40A1 in human cell line U-2 OS using a SLC40A1 Polyclonal Antibody (Product # PA5-64232) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ferroportin in human spleen using Ferroportin Polyclonal Antibody (Product # PA5-64232) shows weak to moderate cytoplasmic positivity in cells in red pulp.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ferroportin in human placenta using Ferroportin Polyclonal Antibody (Product # PA5-64232) shows weak to moderate positivity in erythrocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ferroportin in human duodenum using Ferroportin Polyclonal Antibody (Product # PA5-64232) shows weak cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Ferroportin in human pancreas using Ferroportin Polyclonal Antibody (Product # PA5-64232) shows no cytoplasmic positivity in exocrine glandular cells as expected.
