Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB9924-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ferroportin/SLC40A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Ferroportin/SLC40A1 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9NP59
- Isotype
- IgG
- Antibody clone number
- 1308C
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ferroportin/SLC40A1 in Human Liver. Ferroportin/SLC40A1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Ferroportin/SLC40A1 Monoclonal Antibody (Catalog # MAB9924) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Ferroportin/SLC40A1 in HEK293 Human Cell Line Transfected with Human Ferroportin/SLC40A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Monoclonal Antibody (Catalog # MAB9924) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.