Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 44-800G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-INSR (Tyr972) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references JMJD8 is a Novel Molecular Nexus Between Adipocyte-Intrinsic Inflammation and Insulin Resistance.
Lysophospholipid acylation modulates plasma membrane lipid organization and insulin sensitivity in skeletal muscle.
Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle.
Butyrate Regulates Liver Mitochondrial Function, Efficiency, and Dynamics in Insulin-Resistant Obese Mice.
PTI-125 binds and reverses an altered conformation of filamin A to reduce Alzheimer's disease pathogenesis.
Novel Monoclonal Antibody Is an Allosteric Insulin Receptor Antagonist That Induces Insulin Resistance.
Hypothalamic Ventromedial Lin28a Enhances Glucose Metabolism in Diet-Induced Obesity.
Novel method demonstrates differential ligand activation and phosphatase-mediated deactivation of insulin receptor tyrosine-specific phosphorylation.
Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle.
Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice.
Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.
Altered energy homeostasis and resistance to diet-induced obesity in KRAP-deficient mice.
Insulin enhances growth hormone induction of the MEK/ERK signaling pathway.
You D, Chul Jung B, Villivalam SD, Lim HW, Kang S
Diabetes 2021 Oct 21;
Diabetes 2021 Oct 21;
Lysophospholipid acylation modulates plasma membrane lipid organization and insulin sensitivity in skeletal muscle.
Ferrara PJ, Rong X, Maschek JA, Verkerke AR, Siripoksup P, Song H, Green TD, Krishnan KC, Johnson JM, Turk J, Houmard JA, Lusis AJ, Drummond MJ, McClung JM, Cox JE, Shaikh SR, Tontonoz P, Holland WL, Funai K
The Journal of clinical investigation 2021 Apr 15;131(8)
The Journal of clinical investigation 2021 Apr 15;131(8)
Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle.
Nakazawa H, Ikeda K, Shinozaki S, Yasuhara S, Yu YM, Martyn JAJ, Tompkins RG, Yorozu T, Inoue S, Kaneki M
FEBS open bio 2019 Feb;9(2):348-363
FEBS open bio 2019 Feb;9(2):348-363
Butyrate Regulates Liver Mitochondrial Function, Efficiency, and Dynamics in Insulin-Resistant Obese Mice.
Mollica MP, Mattace Raso G, Cavaliere G, Trinchese G, De Filippo C, Aceto S, Prisco M, Pirozzi C, Di Guida F, Lama A, Crispino M, Tronino D, Di Vaio P, Berni Canani R, Calignano A, Meli R
Diabetes 2017 May;66(5):1405-1418
Diabetes 2017 May;66(5):1405-1418
PTI-125 binds and reverses an altered conformation of filamin A to reduce Alzheimer's disease pathogenesis.
Wang HY, Lee KC, Pei Z, Khan A, Bakshi K, Burns LH
Neurobiology of aging 2017 Jul;55:99-114
Neurobiology of aging 2017 Jul;55:99-114
Novel Monoclonal Antibody Is an Allosteric Insulin Receptor Antagonist That Induces Insulin Resistance.
Cieniewicz AM, Kirchner T, Hinke SA, Nanjunda R, D'Aquino K, Boayke K, Cooper PR, Perkinson R, Chiu ML, Jarantow S, Johnson DL, Whaley JM, Lacy ER, Lingham RB, Liang Y, Kihm AJ
Diabetes 2017 Jan;66(1):206-217
Diabetes 2017 Jan;66(1):206-217
Hypothalamic Ventromedial Lin28a Enhances Glucose Metabolism in Diet-Induced Obesity.
Kim JD, Toda C, Ramírez CM, Fernández-Hernando C, Diano S
Diabetes 2017 Aug;66(8):2102-2111
Diabetes 2017 Aug;66(8):2102-2111
Novel method demonstrates differential ligand activation and phosphatase-mediated deactivation of insulin receptor tyrosine-specific phosphorylation.
Cieniewicz AM, Cooper PR, McGehee J, Lingham RB, Kihm AJ
Cellular signalling 2016 Aug;28(8):1037-47
Cellular signalling 2016 Aug;28(8):1037-47
Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle.
Nakazawa H, Yamada M, Tanaka T, Kramer J, Yu YM, Fischman AJ, Martyn JA, Tompkins RG, Kaneki M
PloS one 2015;10(1):e0116633
PloS one 2015;10(1):e0116633
Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice.
Kim JD, Toda C, D'Agostino G, Zeiss CJ, DiLeone RJ, Elsworth JD, Kibbey RG, Chan O, Harvey BK, Richie CT, Savolainen M, Myöhänen T, Jeong JK, Diano S
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):11876-81
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):11876-81
Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.
Wang HY, Bakshi K, Frankfurt M, Stucky A, Goberdhan M, Shah SM, Burns LH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jul 18;32(29):9773-84
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jul 18;32(29):9773-84
Altered energy homeostasis and resistance to diet-induced obesity in KRAP-deficient mice.
Fujimoto T, Miyasaka K, Koyanagi M, Tsunoda T, Baba I, Doi K, Ohta M, Kato N, Sasazuki T, Shirasawa S
PloS one 2009;4(1):e4240
PloS one 2009;4(1):e4240
Insulin enhances growth hormone induction of the MEK/ERK signaling pathway.
Xu J, Keeton AB, Franklin JL, Li X, Venable DY, Frank SJ, Messina JL
The Journal of biological chemistry 2006 Jan 13;281(2):982-92
The Journal of biological chemistry 2006 Jan 13;281(2):982-92
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Up-regulation and Antibody-Peptide Competition. Upregulation and Antibody-Peptide Competition. Extracts of CHO-T cells transfected with an insulin receptor-containing vector and left unstimulated (1) or stimulated with 50 nM insulin for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the IR (pY972) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to IR (pY972) completely blocks the antibody signal, demonstrating the specificity of the antibody. The data also show up-regulation of the signal upon stimulation with insulin in this cell system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of INSR (pY972) was done on 70% confluent log phase MCF7 cells with insulin treatment (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with INSR (pY972) Rabbit polyclonal Antibody (Product # 44-800G) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing membrane localization. Panel e is untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 FTI-277 treatment reversed burn-induced impaired insulin receptor (IR)-insulin receptor substrate-1 (IRS-1) signaling in skeletal muscle. IR protein expression was not altered by burn, FTI-277 or insulin (B). Insulin-stimulated IR phosphorylation was attenuated at 3 days after burn compared with sham-burned mice, which was almost completely restored by FTI-277 treatment (C, D). Burn decreased IRS-1 protein expression (E) and insulin-stimulated IRS-1 phosphorylation (F, G), both of which were reversed by FTI-277. Insulin significantly increased p-IRS-1/GAPDH ratio and p-IRS-1/IRS-1 ratio in sham-burned mice and FTI-277-treated burned mice, whereas insulin failed to significantly increase p-IRS-1/GAPDH ratio or p-IRS-1/IRS-1 ratio in vehicle-treated burned mice (F, G). There was a trend of increase in insulin-stimulated p-IRS-1/IRS-1 ratio in vehicle-treated burned mice, but there was no statistical difference (G). n = 5 mice per group for saline-injected mice, n = 6 mice per group for insulin-injected sham-burned mice, n = 8 mice per group for insulin-injected burned mice. *p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Lin28a affects AKT-mTOR pathways. A : Western blot images showing p-IR, total IR, p-AKT, total AKT, p-S6K1, total S6K1, and beta-actin in VMH samples of control and Lin28aKI VMH mice on an HFD (45%). B - D : Graphs showing the densitometry analyses of p-IR/IR ( B ), p-AKT/AKT ( C ), and p-S6K1/S6K1 ( D ) in the VMH of Lin28aKI VMH mice and controls. E : Western blot images showing p-IR, total IR, p-AKT, total AKT, p-S6K1, total S6K1, and beta-actin in VMH samples of control and Lin28aKD VMH mice on an HFD (45%). F - H : Graphs showing the densitometry analyses of p-IR/IR ( F ), p-AKT/AKT ( G ), and p-S6K1/S6K1 ( H ) in the VMH of Lin28aKD VMH mice and controls. n = 3 for all groups. Data represent the mean +- SEM. * P < 0.05; ** P < 0.01.