AHR0231

antibody from Invitrogen Antibodies

Targeting: INSR CD220

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AHR0231

Provider

Invitrogen Antibodies

Product name

INSR alpha Monoclonal Antibody (83-7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody is specific for IR and shows no cross-reactivity with insulin-like growth factor (IGF)-receptors. The epitope for this monoclonal antibody is conformational and is located in exon 3. Staining of formalin-fixed, paraffin tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris-HCl, pH 2.0, for 15 min at room temperature or 10 min at 37ºC. Recommended positive controls include IM-9 lymphocytes, placenta, or breast carcinoma.

Reactivity

Human, Bovine, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

83-7

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Regulation of age-associated insulin resistance by MT1-MMP-mediated cleavage of insulin receptor.

Guo X, Asthana P, Gurung S, Zhang S, Wong SKK, Fallah S, Chow CFW, Che S, Zhai L, Wang Z, Ge X, Jiang Z, Wu J, Zhang Y, Wu X, Xu K, Lin CY, Kwan HY, Lyu A, Zhou Z, Bian ZX, Wong HLX
Nature communications 2022 Jun 29;13(1):3749

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Display of Single-Chain Insulin-like Peptides on a Yeast Surface.

Jeong MY, Rutter J, Chou DH
Biochemistry 2019 Jan 22;58(3):182-188

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Sequential cleavage of insulin receptor by calpain 2 and γ-secretase impairs insulin signalling.

Yuasa T, Amo-Shiinoki K, Ishikura S, Takahara M, Matsuoka T, Kaneto H, Kuroda A, Matsuhisa M, Hashida S
Diabetologia 2016 Dec;59(12):2711-2721

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product # AHR0231) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of INSR/Insulin Receptor beta in formalin-fixed, paraffin-embedded human placenta tissue using an INSR monoclonal antibody (Product # AHR0231). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Insulin Receptor alpha in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Insulin Receptor alpha monoclonal antibody (Product # AHR0231) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Insulin Receptor alpha in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Insulin Receptor alpha monoclonal antibody (Product # AHR0231) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.