Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- MAB15442-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Insulin R/CD220 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Insulin R/CD220 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Insulin R/CD220 in NS0 Mouse Cell Line Transfected with Human Insulin R/CD220 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Insulin R/CD220 (filled histogram), or irrelevant transfectant (open histogram) were stained with Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB15442) or Mouse IgG2B Isotype Control (Catalog # MAB0041, data not shown) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Insulin R/CD220 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Mouse Anti-Human Insulin R/CD220 Monoclonal Antibody (Catalog # MAB15442, filled histogram) or Mouse IgG2B Isotype Control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.