RQ5135

antibody from NSJ Bioreagents

Targeting: INSR CD220

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5135

Provider

NSJ Bioreagents

Product name

Insulin Receptor Antibody

Antibody type

Monoclonal

Description

This highly specific Insulin Receptor antibody is suitable for use in Western blot/Immunofluorescence applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

GGA-9

Vial size

100 ul

Concentration

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Storage

Store the Insulin Receptor antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa cell lysate with Insulin Receptor antibody. Expected molecular weight: ~156 kDa (precursor), ~95 kDa (b-subunit).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa cell lysate with Insulin Receptor antibody. Expected molecular weight: ~156 kDa (precursor), ~95 kDa (b-subunit).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with Insulin Receptor antibody. Expected molecular weight: ~156 kDa (precursor), ~95 kDa (b-subunit).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse liver, 2) mouse spleen, 3) mouse lung, 4) mouse kidney, 5) mouse skin, 6) rat liver, 7) rat spleen, 8) rat lung and 9) rat brain tissue lysate with Insulin Receptor antibody. Expected molecular weight: ~156 kDa (precursor), ~95 kDa (b-subunit).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human BxPC-3 cells with Insulin Receptor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.