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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-13764 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-INSR alpha Monoclonal Antibody (83-7), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13764 targets Insulin Receptor alpha in ELISA, FACS, ICC/IF and IHC (P) applications and shows reactivity with Bovine, Human, Ovine, Porcine, Rat and Rabbit samples. The MA5-13764 immunogen is iM-9 lymphocytes followed by IR.
- Reactivity
- Human, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 83-7
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references The signalling conformation of the insulin receptor ectodomain.
The beta secretase BACE1 regulates the expression of insulin receptor in the liver.
A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus.
Inhibition by transmembrane peptides of chimeric insulin receptors.
Functional insulin receptors on human epithelial ovarian carcinoma cells: implications for IGF-II mitogenic signaling.
Weis F, Menting JG, Margetts MB, Chan SJ, Xu Y, Tennagels N, Wohlfart P, Langer T, Müller CW, Dreyer MK, Lawrence MC
Nature communications 2018 Oct 24;9(1):4420
Nature communications 2018 Oct 24;9(1):4420
The beta secretase BACE1 regulates the expression of insulin receptor in the liver.
Meakin PJ, Mezzapesa A, Benabou E, Haas ME, Bonardo B, Grino M, Brunel JM, Desbois-Mouthon C, Biddinger SB, Govers R, Ashford MLJ, Peiretti F
Nature communications 2018 Apr 3;9(1):1306
Nature communications 2018 Apr 3;9(1):1306
A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus.
Umehara A, Nishioka M, Obata T, Ebina Y, Shiota H, Hashida S
Clinical biochemistry 2009 Sep;42(13-14):1468-75
Clinical biochemistry 2009 Sep;42(13-14):1468-75
Inhibition by transmembrane peptides of chimeric insulin receptors.
Bennasroune A, Gardin A, Auzan C, Clauser E, Dirrig-Grosch S, Meira M, Appert-Collin A, Aunis D, Crémel G, Hubert P
Cellular and molecular life sciences : CMLS 2005 Sep;62(18):2124-31
Cellular and molecular life sciences : CMLS 2005 Sep;62(18):2124-31
Functional insulin receptors on human epithelial ovarian carcinoma cells: implications for IGF-II mitogenic signaling.
Kalli KR, Falowo OI, Bale LK, Zschunke MA, Roche PC, Conover CA
Endocrinology 2002 Sep;143(9):3259-67
Endocrinology 2002 Sep;143(9):3259-67
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product # MA5-13767) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human placenta stained with Insulin Receptor antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of trophoblasts.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Insulin Receptor alpha in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Insulin Receptor alpha monoclonal antibody (Product # MA5-13767) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Insulin Receptor alpha in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Insulin Receptor alpha monoclonal antibody (Product # MA5-13767) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
- Conjugate
- Biotin
