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PA5-27334

antibody from Invitrogen Antibodies

Targeting: INSR CD220

Provider product page for PA5-27334

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [5]
Immunohistochemistry [2]

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Validation data

Product number: PA5-27334 - Provider product page
Provider: Invitrogen Antibodies
Product name: INSR Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, NIH-3T3, PC-12, rat liver. Predicted reactivity: Mouse (95%), Rat (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.31 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

INSR protein structure - PA5-27334 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of INSR/Insulin Receptor using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with an INSR/Insulin Receptor polyclonal antibody (Product # PA5-27334) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of INSR was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a INSR Polyclonal Antibody (Product # PA5-27334) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using INSR Polyclonal Antibody (Product # PA5-27334). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 5% SDS PAGE. INSR Polyclonal Antibody (Product # PA5-27334) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of INSR was performed by separating 50 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a INSR Polyclonal Antibody (Product # PA5-27334) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of INSR was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a INSR Polyclonal Antibody (Product # PA5-27334) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.