AM00076PU-N

antibody from Acris Antibodies GmbH

Targeting: INSR CD220

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AM00076PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00076PU-N, RRID:AB_975474

Product name

anti CD220 / INSR pTyr1322 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopeptide conjugated to KLH. Epitope: Phosphotyrosine 1322 (HIPpYTHM)

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

21G12

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Phosphospecificity: Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: untreated Lane 2: Insulin-treated Lane 3: IGF1-treatedLane 4: Pervanadate-treated