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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- HPA036541 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA036541, RRID:AB_2675182
- Product name
- Anti-HIBCH
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human HIBCH, Gene description: 3-hydroxyisobutyryl-CoA hydrolase, Validated applications: ICC, IHC, WB, Uniprot ID: Q6NVY1, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Metabolic role of the hepatic valine/3-hydroxyisobutyrate (3-HIB) pathway in fatty liver disease
Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA Metabolism
3-Hydroxyisobutyrate, A Strong Marker of Insulin Resistance in Type 2 Diabetes and Obesity That Modulates White and Brown Adipocyte Metabolism
Bjune M, Lawrence-Archer L, Laupsa-Borge J, Sommersten C, McCann A, Glastad R, Johnston I, Kern M, Blüher M, Mellgren G, Dankel S
eBioMedicine 2023;91
eBioMedicine 2023;91
Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA Metabolism
Jersin R, Sri Priyanka Tallapragada D, Skartveit L, Bjune M, Muniandy M, Lee-Ødegård S, Heinonen S, Alvarez M, Birkeland K, André Drevon C, Pajukanta P, McCann A, Pietiläinen K, Claussnitzer M, Mellgren G, Dankel S
The Journal of Clinical Endocrinology & Metabolism 2023;108(9):2217-2229
The Journal of Clinical Endocrinology & Metabolism 2023;108(9):2217-2229
3-Hydroxyisobutyrate, A Strong Marker of Insulin Resistance in Type 2 Diabetes and Obesity That Modulates White and Brown Adipocyte Metabolism
Nilsen M, Jersin R, Ulvik A, Madsen A, McCann A, Svensson P, Svensson M, Nedrebø B, Gudbrandsen O, Tell G, Kahn C, Ueland P, Mellgren G, Dankel S
Diabetes 2020;69(9):1903-1916
Diabetes 2020;69(9):1903-1916
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting HIBCH or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting HIBCH Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:376
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HIBCH antibody. Remaining relative intensity is presented.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human kidney and skeletal muscle tissues using Anti-HIBCH antibody. Corresponding HIBCH RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
