TA501316

antibody from OriGene

Targeting: HIBCH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501316

Provider

OriGene

Proper citation

OriGene Cat#TA501316, RRID:AB_11140946

Product name

Anti-HIBCH mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)

Antibody type

Monoclonal

Description

Anti-HIBCH mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)

Host

Mouse

Conjugate

Unconjugated

Epitope

HIBCH

Isotype

IgG

Antibody clone number

OTI3B10

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-HIBCH mouse monoclonal antibody (TA501316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501316), and then analyzed by flow cytometry.

Validation comment

FC