H00003628-M14

antibody from Abnova Corporation

Targeting: INPP1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003628-M14

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003628-M14, RRID:AB_1576354

Product name

INPP1 monoclonal antibody (M14), clone 1A6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant INPP1.

Antigen sequence

MSDILRELLCVSEKAANIARACRQQEALFQLLIEE
KKEGEKNKKFAVDFKTLADVLVQEVIKQNMENKFP
GLEKNIFGEESNEFTNDWGEKITLRLCSTEEETAE
LLSKVLNGNKVASEALARVVHQDVAFTDPTLDSTE
INVPQDILGIWVDPIDSTYQYIKGSADIKSNQGIF
PCGLQCVTILIGVYDIQTGVPLMGVINQPFVSRDP
NTLRWKGQCYWGLSYMGTNMHSLQLTISRRNGSET
HTGNTGSEAAFSPSFSAVISTSEKETIKAALSRVC
GDRIFGAAGAGYKSLCVVQGLVDIYIFSEDTTFKW
DSCAAHAILRAMGGGIVDLKECLERNPETGLDLPQ
LVYHVENEGAAGVDRWANKGGLIAYRSRKRLETFL
SLLVQNLAPAETHT


Isotype

IgG

Antibody clone number

1A6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of INPP1 expression in transfected 293T cell line by INPP1 monoclonal antibody (M14), clone 1A6.Lane 1: INPP1 transfected lysate(44 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged INPP1 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of INPP1 transfected lysate using anti-INPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with INPP1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol