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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- R32030 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CDK2 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids 81-298 of human Cdk2 were used as the immunogen for the Cdk2 antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Cdk2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat kidney, 2) rat liver, 3) human placenta, 4) HeLa, 5) HUT, 6) Jurkat, 7) A549 lysate with Cdk2 antibody. Predicted/observed molecular weight ~33 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Jurkat, 2) HepG2, 3) U-2 OS, 4) K562, 5) T-47D, 6) Caco-2, 7) 293T and 8) PC-3 cell lysate with Cdk2 antibody. Predicted molecular weight ~33 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with Cdk2 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer with Cdk2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse testis with Cdk2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat testis with Cdk2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation tissue with Cdk2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
