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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB9940 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9940, RRID:AB_1671797
- Product name
- CDK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.
Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
Elledge SJ, Spottswood MR
The EMBO journal 1991 Sep;10(9):2653-9
The EMBO journal 1991 Sep;10(9):2653-9
Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.
Giordano A, Lee JH, Scheppler JA, Herrmann C, Harlow E, Deuschle U, Beach D, Franza BR Jr
Science (New York, N.Y.) 1991 Sep 13;253(5025):1271-5
Science (New York, N.Y.) 1991 Sep 13;253(5025):1271-5
Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
Tsai LH, Harlow E, Meyerson M
Nature 1991 Sep 12;353(6340):174-7
Nature 1991 Sep 12;353(6340):174-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of CDK2 polyclonal antibody (Cat # PAB9940) was used at a 1 : 200 dilution to detect CDK2 in asynchronous HeLa cell lysates (run in duplicate).Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel.CDK2 is indicated by an arrowhead as a 32-33 KDa band.Note that multiple isoforms as well as phosphorylated forms of CDK2 may be detected by this antibody.Primary antibody was reacted at room temperature for 1 h.After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining eith CDK2 polyclonal antibody (Cat # PAB9940) was diluted 1 : 500 to detect CDK2 in human skin tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required.The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
