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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- TA502935 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502935, RRID:AB_11124188
- Product name
- CDK2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
- Antibody type
- Monoclonal
- Description
- CDK2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDK2
- Isotype
- IgG
- Antibody clone number
- OTI2D9
- Vial size
- 100 µl
- Concentration
- 0.78 mg/ml
Submitted references Raltitrexed Inhibits HepG2 Cell Proliferation via G0/G1 Cell Cycle Arrest.
Zhao H, Zhang Y, Sun J, Zhan C, Zhao L
Oncology research 2016;23(5):237-48
Oncology research 2016;23(5):237-48
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK2 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CDK2 mouse monoclonal antibody (TA502935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502935).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502935)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-CDK2 antibody(TA502935),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDK2 antibody(TA502935), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody(TA502935),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
