ABIN362119

antibody from antibodies-online

Targeting: CDK2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362119

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN362119, RRID:AB_10953428

Product name

anti-Cyclin-Dependent Kinase 2 (CDK2) (pThr160) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pThr160 (T-Y-T (p) -H-E) derived from Human CDK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pThr160

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

p42, a novel cyclin-dependent kinase-activating kinase in mammalian cells.

Liu Y, Wu C, Galaktionov K
The Journal of biological chemistry 2004 Feb 6;279(6):4507-14

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Inhibition of cdk2 activating phosphorylation by mevastatin.

Ukomadu C, Dutta A
The Journal of biological chemistry 2003 Feb 14;278(7):4840-6

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Kinetic mechanism of activation of the Cdk2/cyclin A complex. Key role of the C-lobe of the Cdk.

Morris MC, Gondeau C, Tainer JA, Divita G
The Journal of biological chemistry 2002 Jun 28;277(26):23847-53

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Effects of phosphorylation of threonine 160 on cyclin-dependent kinase 2 structure and activity.

Brown NR, Noble ME, Lawrie AM, Morris MC, Tunnah P, Divita G, Johnson LN, Endicott JA
The Journal of biological chemistry 1999 Mar 26;274(13):8746-56