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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Flow cytometry [3]
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Validation data
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- Product number
- CF502915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK2 Monoclonal Antibody (OTI2A5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2A5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2 an. Positive lysates LY419741 (100 µg) and LC419741 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2 an. Positive lysates LY419741 (100 µg) and LC419741 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDK2-Knockout 293T cells (KO, Cat# LC810794) were separated by SDS-PAGE and immunoblotted with anti-CDK2 monoclonal antibody TA502915, (1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 14 cell lines by using anti-CDK2 monoclonal antibody. (1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CDK2 mouse monoclonal antibody (TA502915) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494). (1:100)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502915). (1:100)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody (TA502915), and then analyzed by flow cytometry. (1: 100)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-CDK2 antibody (TA502915), (Red), compared to a nonspecific negative control antibody, (Blue). (1:100)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-CDK2 antibody (TA502915), (Red), compared to a nonspecific negative control antibody, (Blue). (1:100)
