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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- TA502893S - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK2 Monoclonal Antibody (OTI4D3), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4D3
- Vial size
- 30 µL
- Concentration
- 0.77 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2. Positive lysates LY419741 (100 µg) and LC419741 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-CDK2 mouse monoclonal antibody (TA502893) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody (TA502893), and then analyzed by flow cytometry.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-CDK2 antibody, (Red), compared to PBS (green) and a nonspecific negative control antibody, (Blue). (1:100)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-CDK2 antibody (TA502893), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
