Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2721 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2721, RRID:AB_10003407
- Product name
- Mouse Monoclonal CDK2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Specific for Cdk2
- Reactivity
- Human, Mouse, Xenopus
- Host
- Mouse
- Antigen sequence
The epitope is at the C-terminus.
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Control of DNA replication by the nucleus/cytoplasm ratio in Xenopus.
Murphy CM, Michael WM
The Journal of biological chemistry 2013 Oct 11;288(41):29382-93
The Journal of biological chemistry 2013 Oct 11;288(41):29382-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDK2 Antibody (AN4.3) [NB100-2721] - Lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, HEK293T human embryonic kidney cell line, and C6 rat glioma cell line were probed with 1:1000 dilution of mouse anti-CDK2 monoclonal (NB100-2721) followed by donkey anti-mouse IgG-HRP secondary antibody (HAF018).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDK2 Antibody (AN4.3) [NB100-2721] - CDK2 was detected in human prostate cancer sections using mouse anti-human CDK2 antibody (NB100-2721) followed by incubation with Anti-Mouse IgG VisuCyte HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).