20170-1-AP

antibody from Proteintech Group

Targeting: GLS GLS1, KIAA0838

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20170-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20170-1-AP, RRID:AB_10665373

Product name

KGA-Specific antibody

Antibody type

Polyclonal

Description

KGA-Specific antibody (Cat. #20170-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

WTAP-Mediated Glutaminase Splicing Bias Suppresses Ferroptosis in Hepatocellular Carcinoma.

Zhu C, Wu K, Wu T, Ma J, Ding B, Jiang N, Du K, Ju G, Xie H, Peng C, Wu J, Zheng S
Cancer communications (London, England) 2026;46:0005

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Glutaminase as a metabolic target of choice to counter acquired resistance to Palbociclib by colorectal cancer cells.

Tarrado-Castellarnau M, Foguet C, Tarragó-Celada J, Palobart M, Hernández-Carro C, Perarnau J, Zodda E, Polat IH, Marin S, Suarez-Bonnet A, Lozano JJ, Yuneva M, Thomson TM, Cascante M
Oncogene 2025 Sep;44(36):3386-3406

---

Directed rescue strategy for enhanced implant osteointegration in aged rats.

Li X, Jiang X, He Y, Xiong H, Huang Q, Xu K, Luo XX, Li K, Tao B, Zha K, Wu J, Liu P, Cai K
Nature communications 2025 Nov 24;16(1):10322

---

A Ratiometric Catalog of Protein Isoform Shifts in the Cardiac Fetal Gene Program.

Han Y, Binti S, Wennersten SA, Pandi B, Ng DCM, Lau E, Lam MPY
bioRxiv : the preprint server for biology 2025 May 31;

---

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival.

Goswami MT, Weh E, Subramanya S, Weh KM, Durumutla HB, Hager H, Miller N, Chaudhury S, Andren A, Sajjakulnukit P, Zhang L, Besirli C, Lyssiotis CA, Wubben TJ
eLife 2025 May 21;13

---

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival.

Goswami MT, Weh E, Subramanya S, Weh KM, Durumutla HB, Hager H, Miller N, Chaudhury S, Andren A, Sajjakulnukit P, Zhang L, Besirli CG, Lyssiotis CA, Wubben TJ
bioRxiv : the preprint server for biology 2025 Feb 25;

---

BIX01294 suppresses PDAC growth through inhibition of glutaminase-mediated glutathione dynamics.

Im SS, Seo J, You JE, Bang HW, Kim Y, Kweon J, Kim Y, Shin DM, Son J
Molecular metabolism 2025 Apr;94:102113

---

O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma.

Fang S, Zhu G, Xie Y, Ding M, Zhen N, Zhu J, Mao S, Tang X, Wu H, Zhang Q, Zhang A, Ni X, Pan Q, Ma J
Cell death discovery 2025 Apr 9;11(1):160

---

mTORC2-driven chromatin cGAS mediates chemoresistance through epigenetic reprogramming in colorectal cancer.

Lv G, Wang Q, Lin L, Ye Q, Li X, Zhou Q, Kong X, Deng H, You F, Chen H, Wu S, Yuan L
Nature cell biology 2024 Sep;26(9):1585-1596

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Glutaminase inhibition in combination with azacytidine in myelodysplastic syndromes: a phase 1b/2 clinical trial and correlative analyses.

DiNardo CD, Verma D, Baran N, Bhagat TD, Skwarska A, Lodi A, Saxena K, Cai T, Su X, Guerra VA, Poigaialwar G, Kuruvilla VM, Konoplev S, Gordon-Mitchell S, Pradhan K, Aluri S, Hackman GL, Chaudhry S, Collins M, Sweeney SR, Busquets J, Rathore AS, Deng Q, Green MR, Grant S, Demo S, Choudhary GS, Sahu S, Agarwal B, Spodek M, Thiruthuvanathan V, Will B, Steidl U, Tippett GD, Burger J, Borthakur G, Jabbour E, Pemmaraju N, Kadia T, Kornblau S, Daver NG, Naqvi K, Short NJ, Garcia-Manero G, Tiziani S, Verma A, Konopleva M
Nature cancer 2024 Oct;5(10):1515-1533

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Deacetylation of GLUD1 maintains the survival of lung adenocarcinoma cells under glucose starvation by inhibiting autophagic cell death.

Hu Q, Sun L, Cheng Z, Wang L, Wan X, Xu J, Cheng J, Wang Z, Yuan Y, Wang K, Han T
Cell insight 2024 Oct;3(5):100186

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Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism.

Baytas O, Davidson SM, Kauer JA, Morrow EM
bioRxiv : the preprint server for biology 2024 Nov 7;

---

Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism.

Baytas O, Davidson SM, Kauer JA, Morrow EM
Molecular brain 2024 Nov 27;17(1):87

---

Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor.

Jablonowski CM, Quarni W, Singh S, Tan H, Bostanthirige DH, Jin H, Fang J, Chang TC, Finkelstein D, Cho JH, Hu D, Pagala V, Sakurada SM, Pruett-Miller SM, Wang R, Murphy A, Freeman K, Peng J, Davidoff AM, Wu G, Yang J
eLife 2024 Mar 15;12

---

Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing is associated with therapeutic response to splicing inhibitor.

Jablonowski C, Quarni W, Singh S, Tan H, Bostanthirige DH, Jin H, Fang J, Chang TC, Finkelstein D, Cho JH, Hu D, Pagala V, Sakurada SM, Pruett-Miller SM, Wang R, Murphy A, Freeman K, Peng J, Davidoff AM, Wu G, Yang J
bioRxiv : the preprint server for biology 2023 Nov 20;

---

Inhibition of glutaminase 1-mediated glutaminolysis improves pathological cardiac remodeling.

Yoshikawa S, Nagao M, Toh R, Shinohara M, Iino T, Irino Y, Nishimori M, Tanaka H, Satomi-Kobayashi S, Ishida T, Hirata KI
American journal of physiology. Heart and circulatory physiology 2022 May 1;322(5):H749-H761

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Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons.

Bando H, Fukuda Y, Watanabe N, Olawale JT, Kato K
Frontiers in cellular and infection microbiology 2021;11:788303

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CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Russo GL, Sonsalla G, Natarajan P, Breunig CT, Bulli G, Merl-Pham J, Schmitt S, Giehrl-Schwab J, Giesert F, Jastroch M, Zischka H, Wurst W, Stricker SH, Hauck SM, Masserdotti G, Götz M
Cell stem cell 2021 Mar 4;28(3):524-534.e7

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Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Beręsewicz-Haller M, Krupska O, Bochomulski P, Dudzik D, Chęcińska A, Hilgier W, Barbas C, Zablocki K, Zablocka B
International journal of molecular sciences 2021 Aug 7;22(16)

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A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues.

Chen Y, Zhao Y, Bajor DL, Wang Z, Selfridge JE
Journal of genetics and genomics = Yi chuan xue bao 2020 Jul 20;47(7):389-395

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Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Li B, Cao Y, Meng G, Qian L, Xu T, Yan C, Luo O, Wang S, Wei J, Ding Y, Yu D
EBioMedicine 2019 Jan;39:239-254

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The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

Acharya KD, Nettles SA, Sellers KJ, Im DD, Harling M, Pattanayak C, Vardar-Ulu D, Lichti CF, Huang S, Edwards DP, Srivastava DP, Denner L, Tetel MJ
eNeuro 2017 Sep-Oct;4(5)

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Metabolic Differences in Glutamine Utilization Lead to Metabolic Vulnerabilities in Prostate Cancer.

Zacharias NM, McCullough C, Shanmugavelandy S, Lee J, Lee Y, Dutta P, McHenry J, Nguyen L, Norton W, Jones LW, Bhattacharya PK
Scientific reports 2017 Nov 23;7(1):16159

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Evidence for Altered Glutamine Metabolism in Human Immunodeficiency Virus Type 1 Infected Primary Human CD4(+) T Cells.

Hegedus A, Kavanagh Williamson M, Khan MB, Dias Zeidler J, Da Poian AT, El-Bacha T, Struys EA, Huthoff H
AIDS research and human retroviruses 2017 Dec;33(12):1236-1247

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T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

Sener Z, Cederkvist FH, Volchenkov R, Holen HL, Skålhegg BS
PloS one 2016;11(7):e0160291

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CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

Masamha CP, Xia Z, Peart N, Collum S, Li W, Wagner EJ, Shyu AB
RNA (New York, N.Y.) 2016 Jun;22(6):830-8

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Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Jacque N, Ronchetti AM, Larrue C, Meunier G, Birsen R, Willems L, Saland E, Decroocq J, Maciel TT, Lambert M, Poulain L, Hospital MA, Sujobert P, Joseph L, Chapuis N, Lacombe C, Moura IC, Demo S, Sarry JE, Recher C, Mayeux P, Tamburini J, Bouscary D
Blood 2015 Sep 10;126(11):1346-56

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Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

Herranz D, Ambesi-Impiombato A, Sudderth J, Sánchez-Martín M, Belver L, Tosello V, Xu L, Wendorff AA, Castillo M, Haydu JE, Márquez J, Matés JM, Kung AL, Rayport S, Cordon-Cardo C, DeBerardinis RJ, Ferrando AA
Nature medicine 2015 Oct;21(10):1182-9

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP(GLS antibody) at dilution of 1:2000

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 20170-1-AP(GLS antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The KGA-Specific antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human KGA-Specific. This antibody recognizes human,mouse,rat antigen. The KGA-Specific antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

20170-1-AP.pdf

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