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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00002744-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002744-M01, RRID:AB_2263330
- Product name
- Mouse Monoclonal Glutaminase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GLS - glutaminase
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SIRT5 regulation of ammonia-induced autophagy and mitophagy.
Control of glutamine metabolism by the tumor suppressor Rb.
Combination of neonatal PolyI:C and adolescent phencyclidine treatments is required to induce behavioral abnormalities with overexpression of GLAST in adult mice.
Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.
Dietary branched chain amino acids ameliorate injury-induced cognitive impairment.
Premature senescence of human endothelial cells induced by inhibition of glutaminase.
Polletta L, Vernucci E, Carnevale I, Arcangeli T, Rotili D, Palmerio S, Steegborn C, Nowak T, Schutkowski M, Pellegrini L, Sansone L, Villanova L, Runci A, Pucci B, Morgante E, Fini M, Mai A, Russo MA, Tafani M
Autophagy 2015;11(2):253-70
Autophagy 2015;11(2):253-70
Control of glutamine metabolism by the tumor suppressor Rb.
Reynolds MR, Lane AN, Robertson B, Kemp S, Liu Y, Hill BG, Dean DC, Clem BF
Oncogene 2014 Jan 30;33(5):556-66
Oncogene 2014 Jan 30;33(5):556-66
Combination of neonatal PolyI:C and adolescent phencyclidine treatments is required to induce behavioral abnormalities with overexpression of GLAST in adult mice.
Hida H, Mouri A, Ando Y, Mori K, Mamiya T, Iwamoto K, Ozaki N, Yamada K, Nabeshima T, Noda Y
Behavioural brain research 2014 Jan 1;258:34-42
Behavioural brain research 2014 Jan 1;258:34-42
Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.
Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 24;109(4):1092-7
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 24;109(4):1092-7
Dietary branched chain amino acids ameliorate injury-induced cognitive impairment.
Cole JT, Mitala CM, Kundu S, Verma A, Elkind JA, Nissim I, Cohen AS
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 5;107(1):366-71
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 5;107(1):366-71
Premature senescence of human endothelial cells induced by inhibition of glutaminase.
Unterluggauer H, Mazurek S, Lener B, Hütter E, Eigenbrodt E, Zwerschke W, Jansen-Dürr P
Biogerontology 2008 Aug;9(4):247-59
Biogerontology 2008 Aug;9(4):247-59
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.
