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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8229 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRUNE Antibody / Exopolyphosphatase PRUNE1
- Antibody type
- Polyclonal
- Description
- This highly specific PRUNE antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PRUNE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293T, 2) human Jurkat, 3) human HEL, 4) human MCF7, 5) rat brain, 6) mouse brain and 7) mouse RAW264.7 cell lysate with PRUNE antibody. Predicted molecular weight ~50 kDa (multiple isoforms) but commonly observed at 50-60 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with PRUNE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human 293T cells with PRUNE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRUNE antibody.
