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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53474PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53474PU-N, RRID:AB_11149138
- Product name
- anti PRUNE (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 367-397aa) of human PRUNE
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PRUNE (arrow) in Jurkat cell line lysates (35ug/lane) using PRUNE antibody Cat.-No. AP53474PU-N (C-term).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in prostate carcinoma (Formalin-fixed, Paraffin-embedded) using PRUNE antibody Cat.-No. AP53474PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for IHC; Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using PRUNE antibody Cat.-No. AP53474PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
