AP1404b

antibody from Abcepta

Targeting: GATA4

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1404b

Provider

Abcepta

Proper citation

Abgent Cat#AP1404b, RRID:AB_2108594

Product name

GATA4 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-GATA4 Antibody (C-term) (Cat.#AP1404b) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) (Cat.#AP1404b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

GATA4 Antibody (C-term) (Cat. #AP1404b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50