Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX113194 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113194, RRID:AB_10726138
- Product name
- GATA4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery.
A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
The prevalence of CTNNB1 mutations in primary aldosteronism and consequences for clinical outcomes.
Multiple gene variations contributed to congenital heart disease via GATA family transcriptional regulation.
Abdelmonem M, Shahin NN, Rashed LA, Amin HAA, Shamaa AA, Shaheen AA
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108584
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108584
A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Lao S, Xu J, Liu Y, Cai S, Lin L, Zhang J, Cai D, Yin S
Molecular medicine reports 2017 Jul;16(1):507-514
Molecular medicine reports 2017 Jul;16(1):507-514
The prevalence of CTNNB1 mutations in primary aldosteronism and consequences for clinical outcomes.
Wu VC, Wang SM, Chueh SJ, Yang SY, Huang KH, Lin YH, Wang JJ, Connolly R, Hu YH, Gomez-Sanchez CE, Peng KY, Wu KD
Scientific reports 2017 Jan 19;7:39121
Scientific reports 2017 Jan 19;7:39121
Multiple gene variations contributed to congenital heart disease via GATA family transcriptional regulation.
Qian Y, Xiao D, Guo X, Chen H, Hao L, Ma X, Huang G, Ma D, Wang H
Journal of translational medicine 2017 Apr 3;15(1):69
Journal of translational medicine 2017 Apr 3;15(1):69
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody (GTX113194) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GATA4 antibody detects GATA4 protein by Western blot analysis.A. 50 μg rat heart lysate/extract 10 % SDS-PAGEGATA4 antibody (GTX113194) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE GTX113194 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE GTX113194 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HepG2 whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 antibody (GTX113194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GATA4(GTX113194) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GATA4 antibody detects GATA4 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. GATA4 antibody (GTX113194) diluted at 1:500.