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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- TA500253 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500253, RRID:AB_2108734
- Product name
- Anti-GATA4 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)
- Antibody type
- Monoclonal
- Description
- Anti-GATA4 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GATA4
- Isotype
- IgG
- Antibody clone number
- OTI1H8
- Vial size
- 100 µl
- Concentration
- 1.1 mg/ml
Submitted references miR-200c and GATA binding protein 4 regulate human embryonic stem cell renewal and differentiation.
Huang HN, Chen SY, Hwang SM, Yu CC, Su MW, Mai W, Wang HW, Cheng WC, Schuyler SC, Ma N, Lu FL, Lu J
Stem cell research 2014 Mar;12(2):338-53
Stem cell research 2014 Mar;12(2):338-53
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (RC210945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of GATA4 protein level by using anti-GATA4 antibody in hESCs.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GATA4 mouse monoclonal antibody (TA500253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4(RC210945).
- Validation comment
- IF
