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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-29663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GATA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 nuclear extract, mouse heart. Predicted reactivity: Mouse (89%), Rat (89%), Xenopus laevis (82%), Dog (96%), Pig (93%), Chicken (85%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Epigenetic Changes Governing Scn5a Expression in Denervated Skeletal Muscle.
C-Kit Cardiac Progenitor Cell Based Cell Sheet Improves Vascularization and Attenuates Cardiac Remodeling following Myocardial Infarction in Rats.
Carreras D, Martinez-Moreno R, Pinsach-Abuin ML, Santafe MM, Gomà P, Brugada R, Scornik FS, Pérez GJ, Pagans S
International journal of molecular sciences 2021 Mar 9;22(5)
International journal of molecular sciences 2021 Mar 9;22(5)
C-Kit Cardiac Progenitor Cell Based Cell Sheet Improves Vascularization and Attenuates Cardiac Remodeling following Myocardial Infarction in Rats.
Dergilev K, Tsokolaeva Z, Makarevich P, Beloglazova I, Zubkova E, Boldyreva M, Ratner E, Dyikanov D, Menshikov M, Ovchinnikov A, Ageev F, Parfyonova Y
BioMed research international 2018;2018:3536854
BioMed research international 2018;2018:3536854
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA4 using 30 µg of A) A549 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GATA4 polyclonal antibody (Product # PA5-29663) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA4 using 30 µg of A) NIH-3T3 (B) JC and C) BCL-1 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GATA4 polyclonal antibody (Product # PA5-29663) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA4 using 50 µg rat heart lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GATA4 polyclonal antibody (Product # PA5-29663) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GATA4 was performed by separating 30 µg of HepG2 whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GATA4 Polyclonal Antibody (Product # PA5-29663) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GATA4 Polyclonal Antibody (Product # PA5-29663). HepG2 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 Polyclonal Antibody (Product # PA5-29663) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GATA4 Polyclonal Antibody (Product # PA5-29663). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA4 Polyclonal Antibody (Product # PA5-29663) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GATA4 in paraformaldehyde-fixed HeLa cells using a GATA4 polyclonal antibody (Product # PA5-29663) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GATA4 was performed in paraffin-embedded mouse duodenum tissue using GATA4 Polyclonal Antibody (Product # PA5-29663) at a dilution of 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GATA4 Polyclonal Antibody detects GATA4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. GATA4 stained by GATA4 Polyclonal Antibody (Product # PA5-29663) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GATA4 Polyclonal Antibody detects GATA4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: GATA4 stained by GATA4 Polyclonal Antibody (Product # PA5-29663) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GATA4 Polyclonal Antibody detects GATA4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GATA4 stained by GATA4 Polyclonal Antibody (Product # PA5-29663) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Isolation and characterization of cell sheets from c-kit+ CPC. (a) CPC sheets formed after 3 days of culture detached at room temperature onto thermoresponsive surfaces yielding a scaffold-free monolayered cell sheet. (b) Hematoxylin-eosin staining shows that the cell sheet represents a well-organized structure. CPC in the cell sheets survived, retained the ability to proliferate (Ki67), expressed progenitor cell marker Gata 4, interconnected with each other via connexin-43 gap junctions, and were surrounded extracellular matrix proteins (collagen 1, collagen 3, and fibronectin). (c) Macroscopic visualization of epicardially attached CPC sheet after implantation.
