Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-21064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZIP10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human spleen tissue lysate. PA5-21064 can be used with blocking peptide PEP-1178.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.
Protic I, Golic I, Vidakovic S, Korac B, Korac A
Current issues in molecular biology 2022 Jul 31;44(8):3444-3454
Current issues in molecular biology 2022 Jul 31;44(8):3444-3454
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescence detection of ZIP10 transporter in normozoospermic ( a ) and asthenozoospermic ( c ) sperm samples using a confocal laser scanning microscope. Sub-compartments of anterior head region: acorn-like ( 1 ), crescent-like ( 2 ), acorn/crescent-like ( 3 ), wreath-like ( 4 ) patterns and posterior head region. Nc--negative control. Scale bar = 10 um. Average of total number of spermatozoa per ejaculate before processing was in normo- (625 x 10 6 /vol) and asthenozoospermia (367.5 x 10 6 /vol); For each type of sample, six randomly selected field areas were analysed (>100 cells). Relative immunopositivity of ZIP10 among the compartments in the normozoospermic ( b ) and asthenozoospermic ( d ) sperm samples. * in comparison to head, # in comparison to midpiece, *** p