703165

antibody from Invitrogen Antibodies

Targeting: TNFAIP8L2 FLJ23467

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703165

Provider

Invitrogen Antibodies

Product name

TNFAIP8L2 Recombinant Rabbit Monoclonal Antibody (19H35L22)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

19H35L22

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, RAW 264.7 cells were fixed and permeabilized for detection of endogenous TNFAIP8L2 using Anti-TNFAIP8L2 Recombinant Rabbit Monoclonal Antibody (Product # 703165, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TNFAIP8L2 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of TNFAIP8L2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of TNFAIP8L2 was achieved by transfecting RAW 264.7 cells with specific siRNA (Silencer® select Product # s35946, s35945). Immunofluorescence analysis was performed on RAW 264.7 cells (untransfected, panel a-d), transfected with TNFAIP8L2 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-TNFAIP8L2 Recombinant Rabbit Monoclonal Antibody (Product # 703165, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to TNFAIP8L2 (green). The images were captured at 60X magnification.