Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AX10010 - Provider product page
- Provider
- Abgent
- Product name
- SRC Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- RB41141
- Vial size
- 400 µl
- Storage
- Store at 2 to 8°C.Antibody is stable for 44 months.
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "SRC Antibody (N-term) (Cat. #AX10010) western blot analysis in MCF-7,A431 cell line lysates (35ug/lane).This demonstrates the SRC antibody detected the SRC protein (arrow)."
- Primary Ab dilution
- 1:2000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SRC Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:50
