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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F52054 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Src Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Src antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Src antibody with A431 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Src antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary Ab was used for the analysis.
