F50715

antibody from NSJ Bioreagents

Targeting: SRC ASV, c-src, SRC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F50715

Provider

NSJ Bioreagents

Product name

c-Src Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this c-Src antibody.

Description

Purified antibody

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the c-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of c-Src antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human breast carcinoma tissue stained with the c-Src antibody