Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9034-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn.
- Antibody clone number
- SC1T2M3
- Concentration
- 0.5 mg/mL
Submitted references Drug conjugated nanoparticles activated by cancer cell specific mRNA.
Hyperreactivity of junctional adhesion molecule A-deficient platelets accelerates atherosclerosis in hyperlipidemic mice.
Lck availability during thymic selection determines the recognition specificity of the T cell repertoire.
Hyperactivated MyD88 signaling in dendritic cells, through specific deletion of Lyn kinase, causes severe autoimmunity and inflammation.
SRC family kinases as potential therapeutic targets for malignancies and immunological disorders.
Gossai NP, Naumann JA, Li NS, Zamora EA, Gordon DJ, Piccirilli JA, Gordon PM
Oncotarget 2016 Jun 21;7(25):38243-38256
Oncotarget 2016 Jun 21;7(25):38243-38256
Hyperreactivity of junctional adhesion molecule A-deficient platelets accelerates atherosclerosis in hyperlipidemic mice.
Karshovska E, Zhao Z, Blanchet X, Schmitt MM, Bidzhekov K, Soehnlein O, von Hundelshausen P, Mattheij NJ, Cosemans JM, Megens RT, Koeppel TA, Schober A, Hackeng TM, Weber C, Koenen RR
Circulation research 2015 Feb 13;116(4):587-99
Circulation research 2015 Feb 13;116(4):587-99
Lck availability during thymic selection determines the recognition specificity of the T cell repertoire.
Van Laethem F, Tikhonova AN, Pobezinsky LA, Tai X, Kimura MY, Le Saout C, Guinter TI, Adams A, Sharrow SO, Bernhardt G, Feigenbaum L, Singer A
Cell 2013 Sep 12;154(6):1326-41
Cell 2013 Sep 12;154(6):1326-41
Hyperactivated MyD88 signaling in dendritic cells, through specific deletion of Lyn kinase, causes severe autoimmunity and inflammation.
Lamagna C, Scapini P, van Ziffle JA, DeFranco AL, Lowell CA
Proceedings of the National Academy of Sciences of the United States of America 2013 Aug 27;110(35):E3311-20
Proceedings of the National Academy of Sciences of the United States of America 2013 Aug 27;110(35):E3311-20
SRC family kinases as potential therapeutic targets for malignancies and immunological disorders.
Benati D, Baldari CT
Current medicinal chemistry 2008;15(12):1154-65
Current medicinal chemistry 2008;15(12):1154-65
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoblotting of reduced lysates from Jurkat T cells left untreated (lane 1), treated with hydrogen peroxide-activated sodium pervanadate (lane 2), or treated with staurosporine (lane 3) using 5 µg/mL of Anti-Human/Mouse phospho-Src (Y418) Purified.