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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- APRab04514 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- c-Src (phospho Tyr529) Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purification
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30‘, using Src （Phospho-Tyr529） Antibody. The lane on the right is blocked with the phospho peptide.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of various cells using Phospho-c-Src （Y529） Polyclonal Antibody diluted at 1：2000
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of COS7 cells using Phospho-c-Src （Y529） Polyclonal Antibody diluted at 1：2000
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using Src （Phospho-Tyr529） Antibody
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src （Phospho-Tyr529） Antibody. The picture on the right is blocked with the phospho peptide.
