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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AHO1402 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin A Monoclonal Antibody (209 No. 13)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 209 No. 13
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Proteins (500 µg) from the breast carcinoma cell line, MDA.MB.231 immunoprecipitated (1 µg/mL) at 4°C with this anti-filamin-A monoclonal antibody (clone Alper-p280) and resolved by SDS-PAGE on 8% polyacrylamide gel and transferred to PVDF
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Filamin A was performed using MDA-MB-231 cells seeded on matrigel. The cells were fixed with formalin and permeabilized with Triton™ X-100. The cells were then labeled with Filamin A Monoclonal Antibody (209 No. 13)(Product # AHO1402) and then with Donkey anti-Mouse Secondary antibody, Texas-Red. Actin was stained with FITC-Phalloidin. The images were captured at 40X magnification using a confocal microscope.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Filamin A was performed using formalin fixed and paraffin-embedded human normal breast (Panel A) and breast cancer (Panel B) tissue. Thin sections were labeled with Filamin A Monoclonal Antibody (209 No. 13)(Product # AHO1402) and then with secondary antibody staining EnVision plus anti-mouse polymer. Panel C and D represents the magnified view of Panel B.
