LS-C750591

antibody from LSBio

Targeting: FLNA ABP-280, FLN, FLN1, OPD1, OPD2

Western blot (Enhanced validation)

Antibody Data

Product number

LS-C750591

Provider

LSBio

Product name

FLNA / Filamin A Antibody (phospho-Ser2152) LS-C750591

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of HeLa and C6 cells, using Phospho-FLNA-S2152 antibody at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of HeLa and C6 cells, using Phospho-FLNA-S2152 antibody at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.