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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin A alpha-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse kidney, PC-12, Rat2. Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia.
Pleines I, Woods J, Chappaz S, Kew V, Foad N, Ballester-Beltrán J, Aurbach K, Lincetto C, Lane RM, Schevzov G, Alexander WS, Hilton DJ, Astle WJ, Downes K, Nurden P, Westbury SK, Mumford AD, Obaji SG, Collins PW, Delerue F, Ittner LM, Bryce NS, Holliday M, Lucas CA, Hardeman EC, Ouwehand WH, Gunning PW, Turro E, Tijssen MR, Kile BT
The Journal of clinical investigation 2017 Mar 1;127(3):814-829
The Journal of clinical investigation 2017 Mar 1;127(3):814-829
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hep G2 . 5% SDS PAGE. Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598). Sample (50 µg of whole cell lysate). Lane A: mouse kidney . 5% SDS PAGE. Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin A antibody detects Filamin A protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.5 % SDS-PAGE. Filamin A antibody Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Filamin an Alpha 2 in methanol-fixed HeLa cells using a Filamin an Alpha 2 polyclonal antibody (Product # PA5-29598) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin A alpha-2 Polyclonal Antibody detects Filamin A protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Filamin A alpha-2 Polyclonal Antibody (Product # PA5-29598) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 TPM4 insufficiency results in altered platelet morphology. ( A and B ) Ultrastructure of platelets from cases 1-II-5 and 1-III-7 carrying the variant, showing the presence of large platelets with numerous vacuoles indicating increased fragility, contrasting the normal discoid platelet appearance in controls. ( A ) Overview. ( B ) Detail. Scale bars: 1 mum. ( C ) Representative electron microscopic pictures illustrating increased size and fragile appearance in Tpm4 Plt53/Plt53 and Tpm4 Plt53/+ compared with Tpm4 +/+ platelets ( n = 2; each sample was pooled from 2 individuals). Scale bars: 1 mum. ( D ) Western blot showing the presence of degraded filamin A and actinin 1 in Tpm4 Plt53/Plt53 and Tpm4 Plt53/+ compared with Tpm4 +/+ platelets. Results are representative of 2 independent experiments. Bottom panel: beta-Actin loading control.
