Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C150087 - Provider product page
- Provider
- LSBio
- Product name
- Anti-TLR4 Antibody (aa24-631) LS-C150087
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 25µg
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- TLR4 in Human PBMCs. TLR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- TLR4 in Human Prostate. TLR4 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).