Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- bs-1021R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-1021R, RRID:AB_10858011
- Product name
- Rabbit Anti-TLR4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- TLR4
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100µl
- Concentration
- 1µg/µl
- Storage
- Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors.
Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis.
Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts.
Chen X, Yu G, Fan S, Bian M, Ma H, Lu J, Jin L
Carbohydrate polymers 2014 May 25;105:113-20
Carbohydrate polymers 2014 May 25;105:113-20
Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis.
Xing S, Zheng F, Zhang W, Wang D, Xing Q
The Journal of international medical research 2014 Jun 12;42(4):958-965
The Journal of international medical research 2014 Jun 12;42(4):958-965
Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts.
Tang L, Zhou XD, Wang Q, Zhang L, Wang Y, Li XY, Huang DM
Archives of oral biology 2011 Oct;56(10):1064-72
Archives of oral biology 2011 Oct;56(10):1064-72
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Mouse macrophages(RAW264.7)labeled with Rabbit Anti TLR4°CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Mouse
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Mouse heart lysates probed with Anti TLR4°CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 90kD. Observed band size:90kD.
- Sample type
- Mouse
- Other comments
- Heart
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Human
- Other comments
- Placenta
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Rat
- Other comments
- Heart