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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2759 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TLR4 in direct ELISAs. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9QUK6- Isotype
- IgG
- Antibody clone number
- 267518
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells.
Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1 G93A mouse model of amyotrophic lateral sclerosis.
Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation.
Toll-like receptor 4 regulates early endothelial activation during ischemic acute kidney injury.
Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
Stojanovic B, Jovanovic I, Stojanovic BS, Stojanovic MD, Gajovic N, Radosavljevic G, Pantic J, Arsenijevic N, Lukic ML
European journal of immunology 2019 Jun;49(6):940-946
European journal of immunology 2019 Jun;49(6):940-946
Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1 G93A mouse model of amyotrophic lateral sclerosis.
Lee JY, Lee JD, Phipps S, Noakes PG, Woodruff TM
Journal of neuroinflammation 2015 May 13;12:90
Journal of neuroinflammation 2015 May 13;12:90
Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
Yano J, Palmer GE, Eberle KE, Peters BM, Vogl T, McKenzie AN, Fidel PL Jr
Infection and immunity 2014 Feb;82(2):783-92
Infection and immunity 2014 Feb;82(2):783-92
Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation.
Mussalem JS, Squaiella-Baptistão CC, Teixeira D, Yendo TM, Thies FG, Popi AF, Mariano M, Longo-Maugéri I
PloS one 2012;7(3):e33955
PloS one 2012;7(3):e33955
Toll-like receptor 4 regulates early endothelial activation during ischemic acute kidney injury.
Chen J, John R, Richardson JA, Shelton JM, Zhou XJ, Wang Y, Wu QQ, Hartono JR, Winterberg PD, Lu CY
Kidney international 2011 Feb;79(3):288-99
Kidney international 2011 Feb;79(3):288-99
Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
American journal of physiology. Regulatory, integrative and comparative physiology 2009 Dec;297(6):R1670-80
American journal of physiology. Regulatory, integrative and comparative physiology 2009 Dec;297(6):R1670-80
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TLR4 in RAW 264.7 Mouse Monocyte/Macrophage Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line using Rat Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB2759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
