Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB11281 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11281, RRID:AB_1715981
- Product name
- TLR4 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to N-terminus of human TLR4.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Involvement of PKCalpha/beta in TLR4 and TLR2 dependent activation of NF-kappaB.
The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction.
The Toll-IL-1 receptor adaptor family grows to five members.
Asehnoune K, Strassheim D, Mitra S, Yeol Kim J, Abraham E
Cellular signalling 2005 Mar;17(3):385-94
Cellular signalling 2005 Mar;17(3):385-94
The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction.
McGettrick AF, O'Neill LA
Molecular immunology 2004 Jul;41(6-7):577-82
Molecular immunology 2004 Jul;41(6-7):577-82
The Toll-IL-1 receptor adaptor family grows to five members.
O'Neill LA, Fitzgerald KA, Bowie AG
Trends in immunology 2003 Jun;24(6):286-90
Trends in immunology 2003 Jun;24(6):286-90
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using TLR4 polyclonal antibody (Cat # PAB11281) shows detection of TLR4 in PC-3 whole cell lysate.The membrane was probed with the primary antibody diluted to 1 : 400 (A) and 1 : 200 (B).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using TLR4 polyclonal antibody (Cat # PAB11281) shows staining of TLR4 in K-562 cells.The cells were paraformaldehyde fixed and reacted with the primary antibody diluted to 1:500.
- Validation comment
- Immunocytochemistry