PA1484

antibody from Boster Biological Technology

Targeting: TLR4 ARMD10, CD284, hToll, TLR-4

Provider product page for PA1484

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [69]
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Product number: PA1484 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-Toll-like receptor 4 TLR4 Antibody
Antibody type: Polyclonal
Description: Polyclonal antibody for TLR4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. TLR4 information: Molecular Weight: 95680 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis; Tissue Specificity: Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Reactivity: Human
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

TLR4 protein structure - PA1484 shown in red.

Submitted references Network pharmacology combined with molecular docking and experimental validation to explore the potential mechanism of Cinnamomi ramulus against ankylosing spondylitis.

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Annals of medicine 2023;55(2):2287193

Total glycosides of Rhodiola rosea L. attenuate LPS-induced acute lung injury by inhibiting TLR4/NF-κB pathway.

Jia X, Zhang K, Feng S, Li Y, Yao D, Liu Q, Liu D, Li X, Huang J, Wang H, Wang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Feb;158:114186

A study of diabetes-induced erectile dysfunction treated with human umbilical cord mesenchymal stem cells.

Wang S, Zhang A, Liu K, Pan Y, Kang J, Niu S, Song Y, Zhang Z, Li Y, Liu L, Liu X
Andrologia 2022 Aug;54(7):e14440

Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation.

Wang W, Li SS, Xu XF, Yang C, Niu XG, Yin SX, Pan XY, Xu W, Hu GD, Wang C, Liu SW
Acta pharmacologica Sinica 2022 Apr;43(4):771-780

Exploring the Mechanism of Resveratrol in Reducing the Soft Tissue Damage of Osteoarthritis Based on Network Pharmacology and Experimental Pharmacology.

Long Z, Xiang W, Li J, Yang T, Yu G
Evidence-based complementary and alternative medicine : eCAM 2021;2021:9931957

Serum‑derived exosomes from house dust mite‑sensitized guinea pigs contribute to inflammation in BEAS‑2B cells via the TLR4‑NF‑κB pathway.

Liu C, Huang XL, Liang JP, Zhong X, Wei ZF, Dai LX, Wang J
Molecular medicine reports 2021 Nov;24(5)

Protective Effects of Selenium Nanoparticle-Enriched Lactococcus lactis NZ9000 against Enterotoxigenic Escherichia coli K88-Induced Intestinal Barrier Damage in Mice.

Chen Y, Qiao L, Song X, Ma L, Dou X, Xu C
Applied and environmental microbiology 2021 Nov 10;87(23):e0163621

N-acetylserotonin alleviated the expression of interleukin-1β in retinal ischemia-reperfusion rats via the TLR4/NF-κB/NLRP3 pathway.

Liu J, Zhang N, Zhang M, Yin H, Zhang X, Wang X, Wang X, Zhao Y
Experimental eye research 2021 Jul;208:108595

Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner.

Xia D, Yang L, Li Y, Chen J, Zhang X, Wang H, Zhai S, Jiang X, Meca G, Wang S, Huang L, Zhu S, Fu Y, Ma W, Zhu Y, Ye H, Wang W
Journal of hazardous materials 2021 Jul 5;413:125239

Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy.

Xing T, Luo D, Zhao X, Xu X, Li J, Zhang L, Gao F
Journal of the science of food and agriculture 2021 Jan 15;101(1):279-286

HMGB1 is a key factor for tamoxifen resistance and has the potential to predict the efficacy of CDK4/6 inhibitors in breast cancer.

Zhang H, Wang J, Li J, Zhou X, Yin L, Wang Y, Gu Y, Niu X, Yang Y, Ji H, Zhang Q
Cancer science 2021 Apr;112(4):1603-1613

Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis.

Feng S, Ju L, Shao Z, Grzanna M, Jia L, Liu M
Journal of inflammation research 2020;13:343-356

Autophagy inhibits TLR4-mediated invasiveness of oral cancer cells via the NF-κB pathway.

Kong Q, Liang Y, He Q, You Y, Wu L, Liang L, Liang J
Oral diseases 2020 Sep;26(6):1165-1174

Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways.

Shi D, Chen M, Liu L, Wang Q, Liu S, Wang L, Wang R
Journal of ethnopharmacology 2020 May 10;253:112634

Cryptochlorogenic acid attenuates LPS-induced inflammatory response and oxidative stress via upregulation of the Nrf2/HO-1 signaling pathway in RAW 264.7 macrophages.

Zhao XL, Yu L, Zhang SD, Ping K, Ni HY, Qin XY, Zhao CJ, Wang W, Efferth T, Fu YJ
International immunopharmacology 2020 Jun;83:106436

Effect of seasonal variance on intestinal epithelial barriers and the associated innate immune response of the small intestine of the Chinese soft-shelled turtles.

Shi Y, Vistro WA, Bai X, Wu R, Chen C, Huang Y, Fazlani SA, Tarique I, Yang P, Chen Q
Fish & shellfish immunology 2020 Feb;97:173-181

Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway.

Han X, Chen X, Chen S, Luo Q, Liu X, He A, He S, Qiu J, Chen P, Wu Y, Zhuang J, Yang M, Wu C, Wu N, Yang Y, Ge J, Zhuang J, Yu K
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Aug;128:110273

HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway.

Wang F, Ji S, Wang M, Liu L, Li Q, Jiang F, Cen J, Ji B
European journal of pharmacology 2020 Aug 5;880:173189

Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell.

Yang K, Lu T, Zhan L, Zhou C, Zhang N, Lei S, Wang Y, Yang J, Yan M, Lv G, Chen S
International journal of biological macromolecules 2020 Apr 15;149:320-330

A Study of the Immunoregulatory Function of TLR3 and TLR4 on Mesenchymal Stem Cells in Ankylosing Spondylitis.

Li Y, Huang L, Cai Z, Deng W, Wang P, Su H, Wu Y, Shen H
Stem cells and development 2019 Oct 15;28(20):1398-1412

Ochratoxin A induces liver inflammation: involvement of intestinal microbiota.

Wang W, Zhai S, Xia Y, Wang H, Ruan D, Zhou T, Zhu Y, Zhang H, Zhang M, Ye H, Ren W, Yang L
Microbiome 2019 Nov 28;7(1):151

Red yeast rice ameliorates high-fat diet-induced atherosclerosis in Apoe(-/-) mice in association with improved inflammation and altered gut microbiota composition.

Dong Y, Cheng H, Liu Y, Xue M, Liang H
Food & function 2019 Jul 17;10(7):3880-3889

Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll-like receptor-4/nuclear factor-κB signaling pathway.

Bai S, Zhou H, Wu L
Experimental and therapeutic medicine 2019 Jan;17(1):444-448

H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway.

Liu H, Ye T, Yang X, Liu J, Jiang K, Lu H, Xia D, Peng E, Chen Z, Sun F, Tang K, Ye Z
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Effects of the combination of tanshinone IIA and puerarin on cardiac function and inflammatory response in myocardial ischemia mice.

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Journal of molecular and cellular cardiology 2019 Dec;137:59-70

Effect of curcumin supplementation on TLR4 mediated non-specific immune responses in liver of laying hens under high-temperature conditions.

Nawab A, Li G, An L, Wu J, Chao L, Xiao M, Zhao Y, Birmani MW, Ghani MW
Journal of thermal biology 2019 Aug;84:384-397

Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness.

Jin M, Zhang H, Zhao K, Xu C, Shao D, Huang Q, Shi J, Yang H
Frontiers in physiology 2018;9:729

Hypoglycemic Mechanism of the Berberine Organic Acid Salt under the Synergistic Effect of Intestinal Flora and Oxidative Stress.

Cui HX, Hu YN, Li JW, Yuan K
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Expression of Toll‑like receptor 4 on mast cells in gingival tissues of human chronic periodontitis.

Huang B, Dai Q, Huang SG
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Yang L, Wang Y, Nuerbiye A, Cheng P, Wang JH, Kasimu R, Li H
International journal of molecular sciences 2018 May 29;19(6)

Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling.

Gong J, Guan L, Tian P, Li C, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research 2018 May 27;24:3514-3523

The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats.

Zhang Y, Fu LT, Tang F
Molecular medicine reports 2018 Mar;17(3):3455-3464

Lipopolysaccharide enhances TGF-β1 signalling pathway and rat pancreatic fibrosis.

Sun L, Xiu M, Wang S, Brigstock DR, Li H, Qu L, Gao R
Journal of cellular and molecular medicine 2018 Apr;22(4):2346-2356

Regulation of angiotensin II-induced B-cell lymphoma-2-associated athanogene 3 expression in vascular smooth muscle cells.

Yu S, Chen Y, Chen S, Ye N, Li Y, Sun Y
Molecular medicine reports 2018 Apr;17(4):6156-6162

Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens.

Wang L, Jiao H, Zhao J, Wang X, Sun S, Lin H
Frontiers in immunology 2017;8:1856

Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells.

Li H, He B, Liu X, Li J, Liu Q, Dong W, Xu Z, Qian G, Zuo H, Hu C, Qian H, Mao C, Wang G
Theranostics 2017;7(9):2537-2554

Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury.

Shanshan Y, Beibei J, Li T, Minna G, Shipeng L, Li P, Yong Z
Frontiers in cellular neuroscience 2017;11:99

TLR4 signaling is activated in ventilator-induced diaphragm dysfunction in rats.

Liu P, Zhang H, Hu K, Zheng H
International journal of clinical and experimental pathology 2017;10(8):8515-8519

Orientin Attenuates Cerebral Ischemia/Reperfusion Injury in Rat Model through the AQP-4 and TLR4/NF-κB/TNF-α Signaling Pathway.

Wang X, An F, Wang S, An Z, Wang S
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2017 Oct;26(10):2199-2214

Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner.

Sun L, Li M, Ma X, Feng H, Song J, Lv C, He Y
Journal of neuroinflammation 2017 Nov 25;14(1):231

Preventive and therapeutic effect of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.

Zhou HX, Gao LH, Meng LL, Zhang YX, Wei ZF, Si DW
Asian Pacific journal of tropical medicine 2017 Feb;10(2):152-156

Characterization of acetaminophen-induced cytotoxicity in target tissues.

Guo C, Xie G, Su M, Wu X, Lu X, Wu K, Wei C
American journal of translational research 2016;8(10):4440-4445

Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophages.

Zhao LX, Du JR, Zhou HJ, Liu DL, Gu MX, Long FY
PloS one 2016;11(10):e0164586

Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice.

Fei ZW, Zhang XP, Zhang J, Huang XM, Wu DJ, Bi HH
Chinese journal of integrative medicine 2016 Sep;22(9):674-84

Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft-Shelled Turtle Pelodiscus sinensis During the Hibernation Season.

Hu L, Li Q, Yang P, Gandahi JA, Arain TS, Le Y, Zhang Q, Liu T, Y Waqas M, Ahmad N, Liu Y, Chen Q
Anatomical record (Hoboken, N.J. : 2007) 2016 Nov;299(11):1578-1584

Knockdown of versican V1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-κB signaling pathway in C57BL/6J mice.

Xu L, Xue T, Zhang J, Qu J
Molecular medicine reports 2016 Jun;13(6):5005-12

Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir.

Zhou H, He Z, Wang C, Xie T, Liu L, Liu C, Song F, Ma Y
International journal of molecular sciences 2016 Jun 6;17(6)

Prognostic value of toll-like receptor 4 and nuclear factor-κBp65 in oral squamous cell carcinoma patients.

Yang J, Liu D, Khatri KS, Wang J, Zhang G, Meng C, Guan J
Oral surgery, oral medicine, oral pathology and oral radiology 2016 Dec;122(6):753-764.e1

Up-regulated TLR2 and TLR4 expressions in liver and spleen during acute murine T. gondii infection.

Peng J, Lin X, Lin H, Chen S, Liu J, Guo Z, Liang Y, Huang S, Lu F
Parasitology research 2016 Dec;115(12):4681-4686

Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways.

Yue L, Wang W, Wang Y, Du T, Shen W, Tang H, Wang Y, Yin H
International journal of biological macromolecules 2016 Aug;89:376-88

High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy.

Wang L, Wang J, Fang J, Zhou H, Liu X, Su SB
Diabetology & metabolic syndrome 2015;7:89

Expression of TLR2/4 in the sperm-storing oviduct of the Chinese soft-shelled turtle Pelodiscus sinensis during hibernation season.

Li Q, Hu L, Yang P, Zhang Q, Waqas Y, Liu T, Zhang L, Wang S, Chen W, Le Y, Ullah S, Chen Q
Ecology and evolution 2015 Oct;5(19):4466-79

Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis.

Xu M, Wang KN, Wu K, Wang XP
Gut and liver 2015 May 23;9(3):411-6

Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus.

Li X, Qian D, Ju F, Wang B
Oncology letters 2015 Jan;9(1):365-370

Toll-like receptor-2 and -4 are associated with hyperlipidemia.

Zhu YJ, Wang C, Song G, Zang SS, Liu YX, Li L
Molecular medicine reports 2015 Dec;12(6):8241-6

Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance.

Wang YH, Cao YW, Yang XC, Niu HT, Sun LJ, Wang XS, Liu J
Asian Pacific journal of cancer prevention : APJCP 2014;15(3):1321-6

The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation.

Cui L, Feng L, Zhang ZH, Jia XB
International immunopharmacology 2014 Nov;23(1):294-303

Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

Liu MW, Wang YH, Qian CY, Li H
International journal of molecular medicine 2014 Dec;34(6):1492-504

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.

Zhou F, Wang L, Liu P, Hu W, Zhu X, Shen H, Yao Y
Neural regeneration research 2014 Dec 1;9(23):2074-80

TLR4 is constitutively expressed in chick thymic epithelial cells.

Huang HB, Xiang QH, Wu H, Ansari AR, Wen L, Ge XH, Wang JX, Peng KM, Liu HZ
Veterinary immunology and immunopathology 2014 Apr 15;158(3-4):182-8

Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients.

Wang YZ, Tian FF, Liu H, Zhang W, Li J, Xiao B, Zhou WB
Journal of neuroimmunology 2013 Apr 15;257(1-2):67-75

Atorvastatin decreases Toll-like receptor 4 expression and downstream signaling in human monocytic leukemia cells.

Yang SS, Li R, Qu X, Fang W, Quan Z
Cellular immunology 2012 Sep;279(1):96-102

Salvianolic acid B inhibits the TLR4-NFκB-TNFα pathway and attenuates neonatal rat cardiomyocyte injury induced by lipopolysaccharide.

Wang J, Zhang Y, Guo LL, Wu GJ, Liu RH
Chinese journal of integrative medicine 2011 Oct;17(10):775-9

Strategic localization of toll-like receptor 4 in the digestive tract of blunt snout bream (Megalobrama amblycephala).

Zhang GY, Wang Y, Peng KM, Wang C, Tian G, Liu HZ
Anatomia, histologia, embryologia 2009 Nov;38(6):401-5

Preventive effects of Schistosoma japonicum ova on trinitrobenzenesulfonic acid-induced colitis and bacterial translocation in mice.

Zhao Y, Zhang S, Jiang L, Jiang J, Liu H
Journal of gastroenterology and hepatology 2009 Nov;24(11):1775-80

Effect of salvia miltiorrhizae on the expressions of TLR4 protein in the liver of rats with SAP or OJ.

Zhang X, Liu D, Wu D, Zhu C, Ye J, Wang K, Peng L, Zhuo G
Inflammation 2009 Jun;32(3):151-62

Effects of Salvia miltiorrhizae on ICAM-1, TLR4, NF-kappaB and Bax proteins expression in multiple organs of rats with severe acute pancreatitis or obstructive jaundice.

Xiping Z, Dijiong W, Jianfeng L, Qihui C, Jing Y, Penghui J, Meijuan Y, Ninni Z
Inflammation 2009 Aug;32(4):218-32

Effects of trefoil peptide 3 on expression of TNF-alpha, TLR4, and NF-kappaB in trinitrobenzene sulphonic acid induced colitis mice.

Teng X, Xu LF, Zhou P, Sun HW, Sun M
Inflammation 2009 Apr;32(2):120-9

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Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Anti-TLR4 antibody, PA1484, Western blottingLane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate
Additional image