Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1478 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TLR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse TLR4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O00206
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inositol-Requiring Enzyme 1-Mediated Downregulation of MicroRNA (miR)-146a and miR-155 in Primary Dermal Fibroblasts across Three TNFRSF1A Mutations Results in Hyperresponsiveness to Lipopolysaccharide.
Compound A attenuates toll-like receptor 4-mediated paclitaxel resistance in breast cancer and melanoma through suppression of IL-8.
HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.
TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
The C-terminal disulfide bonds of Helicobacter pylori GroES are critical for IL-8 secretion via the TLR4-dependent pathway in gastric epithelial cells.
Regulation of the innate immune response by fibronectin: synergism between the III-1 and EDA domains.
Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
Serum amyloid A in the placenta and its role in trophoblast invasion.
Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.
Slit2-Robo4 pathway modulates lipopolysaccharide-induced endothelial inflammation and its expression is dysregulated during endotoxemia.
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice.
Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Harrison SR, Scambler T, Oubussad L, Wong C, Wittmann M, McDermott MF, Savic S
Frontiers in immunology 2018;9:173
Frontiers in immunology 2018;9:173
Compound A attenuates toll-like receptor 4-mediated paclitaxel resistance in breast cancer and melanoma through suppression of IL-8.
Sootichote R, Thuwajit P, Singsuksawat E, Warnnissorn M, Yenchitsomanus PT, Ithimakin S, Chantharasamee J, Thuwajit C
BMC cancer 2018 Feb 27;18(1):231
BMC cancer 2018 Feb 27;18(1):231
HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.
Ben Haij N, Planès R, Leghmari K, Serrero M, Delobel P, Izopet J, BenMohamed L, Bahraoui E
PloS one 2015;10(6):e0129425
PloS one 2015;10(6):e0129425
TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.
Avlas O, Bragg A, Fuks A, Nicholson JD, Farkash A, Porat E, Aravot D, Levy-Drummer RS, Cohen C, Shainberg A, Arad M, Hochhauser E
PloS one 2015;10(6):e0120175
PloS one 2015;10(6):e0120175
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Vogel S, Börger V, Peters C, Förster M, Liebfried P, Metzger K, Meisel R, Däubener W, Trapp T, Fischer JC, Gawaz M, Sorg RV
Cell death and differentiation 2015 Jul;22(7):1219-30
Cell death and differentiation 2015 Jul;22(7):1219-30
The C-terminal disulfide bonds of Helicobacter pylori GroES are critical for IL-8 secretion via the TLR4-dependent pathway in gastric epithelial cells.
Su YL, Yang JC, Lee H, Sheu F, Hsu CH, Lin SL, Chow LP
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3997-4007
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3997-4007
Regulation of the innate immune response by fibronectin: synergism between the III-1 and EDA domains.
Kelsh R, You R, Horzempa C, Zheng M, McKeown-Longo PJ
PloS one 2014;9(7):e102974
PloS one 2014;9(7):e102974
Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
Alizadeh H, Tripathi T, Abdi M, Smith AD
PloS one 2014;9(3):e92375
PloS one 2014;9(3):e92375
Serum amyloid A in the placenta and its role in trophoblast invasion.
Sandri S, Urban Borbely A, Fernandes I, de Oliveira EM, Knebel FH, Ruano R, Zugaib M, Filippin-Monteiro F, Bevilacqua E, Campa A
PloS one 2014;9(3):e90881
PloS one 2014;9(3):e90881
Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.
Mogami H, Keller PW, Shi H, Word RA
The Journal of biological chemistry 2014 May 9;289(19):13295-307
The Journal of biological chemistry 2014 May 9;289(19):13295-307
Slit2-Robo4 pathway modulates lipopolysaccharide-induced endothelial inflammation and its expression is dysregulated during endotoxemia.
Zhao H, Anand AR, Ganju RK
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):385-93
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):385-93
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Müller I, Mack A, Schumann S, Bühring HJ, Lang F, Sorg RV, Langer H, Gawaz M
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice.
Mogami H, Kishore AH, Shi H, Keller PW, Akgul Y, Word RA
The Journal of biological chemistry 2013 Jan 18;288(3):1953-66
The Journal of biological chemistry 2013 Jan 18;288(3):1953-66
Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
The British journal of dermatology 2011 Aug;165(2):302-10
The British journal of dermatology 2011 Aug;165(2):302-10
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
Clinical and experimental immunology 2009 May;156(2):285-93
Clinical and experimental immunology 2009 May;156(2):285-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TLR4 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1478) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for TLR4 at approximately 110 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TLR4 in Human PBMCs. TLR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TLR4 in Human Prostate. TLR4 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1478) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-8 Secretion Induced by LPS and Neutralization by Human TLR4 Antibody. Lipopolysacharide (LPS) stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line co-transfected with human TLR4 and MD-2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by LPS (75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1478). The ND50 is typically 1.5-7.5 µg/mL.