Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00006707-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006707-M01, RRID:AB_2195271
- Product name
- Mouse Monoclonal SPRR3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPRR3 - small proline-rich protein 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry.
Igoh A, Doi Y, Sakurada K
Analytical and bioanalytical chemistry 2015 Sep;407(23):7135-44
Analytical and bioanalytical chemistry 2015 Sep;407(23):7135-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of SPRR3 expression in transfected 293T cell line by SPRR3 monoclonal antibody (M01), clone 4A12.Lane 1: SPRR3 transfected lysate(18.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SPRR3 Antibody (4A12) [H00006707-M01] - Detection limit for recombinant GST tagged SPRR3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of monoclonal antibody to SPRR3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.