Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C287467 - Provider product page
- Provider
- LSBio
- Product name
- Anti-RPS6 / Ribosomal Protein S6 Antibody (phospho-Ser235) LS-C287467
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Phospho RPS6 (S235) by Western Blot. Samples: Whole cell lysate (35 ug) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235) antibody used at 0.1 ug/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).