Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX12864 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX12864, RRID:AB_367025
- Product name
- RPS6 (phospho Ser235/236) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Activation of the mTOR pathway by low levels of xenoestrogens in breast epithelial cells from high-risk women.
Goodson WH 3rd, Luciani MG, Sayeed SA, Jaffee IM, Moore DH 2nd, Dairkee SH
Carcinogenesis 2011 Nov;32(11):1724-33
Carcinogenesis 2011 Nov;32(11):1724-33
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5). were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 [pSpS235/236] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab¡¦)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to ribosomal protein S6 [pSpS235/236] blocks the signal, verifying the specificity of the antibody.
